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November 21, 2009, 07:33:42 PM
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Author Topic: differentiating Helicoma species  (Read 1259 times)
SRA
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« on: October 30, 2008, 07:59:23 AM »
Hi Everyone,

Do any of you know how to differentiate Helicoma chlamydosporum from Helicoma depressispora?

Any help would be greatly appreciated.

SRA
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BLW
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« Reply #1 on: November 05, 2008, 10:42:59 PM »
Ribosomal sequencing. Go to NCBI and search the nucleotide database with your strain of interest. Look for the ITS or D1/D2 sequence (partial 28S), and pull that out. Run a BLAST search with it--the sequence will find itself, and will find the next closest sequences. if it is far enough away in % identity (>99%), then this could work.
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